Agarose-Out DNA Purification Kit

GeneMATRIX Agarose-Out DNA Purification Kit is designed to isolate ultrapure linear or circular DNA molecules, ranging in size from approximately 100 bp to 10 kb, from TAE- or TBE-agarose gels. It is also possible to purify DNA fragments up to 20 kb or more, with gradually decreasing yields. Coloured solubilizing buffer helps both in monitoring agarose dissolving and in simultaneous processing of multiple samples. Besides agarose many other contaminants are effectively removed: ethidium bromide, RNA, primers, enzymes and other proteins, lipids, endotoxins, dyes, detergents, nucleotides, radio- and chemical labels, EDTA, problematic restriction and ligation inhibitors, buffers and salts. Optimized buffer is added to provide selective conditions for DNA binding during brief centrifugation, while contaminants pass through the GeneMATRIX in the spin-column. Traces of solubilized agarose and other contaminants remaining on the membrane are efficiently removed in two wash steps. The membrane used is particularly designed toward removal of problematic inhibitors of restriction and ligation of DNA. High-quality DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.

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