NucleoBond BAC 100 kit for large construct plasmid DNA

1st generation anion exchange technology for large construct plasmid DNA

Application: Large contruct (P1, BACs, PACs) plasmid purification
Target: Plasmid DNA
CE-zertifiziert: No, research use only
Technology: Anion exchange chromatography
Brand: NucleoBond
Format: BAC prep
Verpackungseinheit: 10 Prep(s)
Handling: Gravity flow
Lysate clarification: Folded filter
Automated use. No
Sample material: Bacteria, E. coli
Sample amount: 100−500 mL E. coli culture
Vector size: < 300 kbp
Typical yield: 10−100 µg
Theoretical binding capacity: 100 µg
Typical purity A260/A280: 1.8−1.95
Endotoxin level: 1−10 EU/µg DNA
Isopropanol precipitation: Centrifugation
Preparation time: 120 min/2−4 preps
Typical downstream application: Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Lagertemperatur: 18−25 °C
Haltbarkeit (ab Produktion): 39 Monate
Lieferumfang: NucleoBond BAC 100 Columns, NucleoBond Folded Filters, buffers, RNase A
Gefahrstoffe: Ja