NucleoSpin TriPrep, Mini kit for RNA, DNA, and protein purification

Parallel isolation of RNA, DNA and, protein from an undivided sample

Application: Isolation of RNA, DNA, and protein
Target: RNA
CE-zertifiziert: No, research use only
Technology: Silica membrane technology
Brand: NucleoSpin
Format: Mini prep
Verpackungseinheit: 10 Prep(s), 50 Prep(s), 250 Prep(s)
Handling: Centrifugation
Lysate clarification: Centrifugation, NucleoSpin Filter
Automated use: No
Sample material: Animal tissue, Cells, Human tissue, Plant
Sample amount: < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue
Fragment size: RNA > 200 nt, DNA < 30 kbp, protein 15–300 kDa
Typical yield: < 70 µg RNA, < 6 µg DNA, < 1200 µg Protein
Theoretical binding capacity: 200 µg
Typical purity A260/A280: RNA 1.9−2.1, DNA 1.7−1.9
Typical RIN: > 9
Elutionsvolumen: RNA 40–120 µL, DNA 100 µL, Protein 10–100 µL
Preparation time: 30 min/6 preps (RNA), 45 min/6 preps (RNA and DNA), + 35 min/6 preps (protein)
Typical downstream application: Chip hybridization, qRT−PCR, RNA-Seq
Lagertemperatur: 18−25 °C
Haltbarkeit (ab Produktion): 24 Monate
Gefahrstoffe: Ja