NucleoBond HMW DNA, high molecular weight DNA from diverse sample materials

Anion exchange chromatography for purification of high integrity DNA from different sample materials

Application: Isolation of DNA
Target: DNA
CE-zertifizier: No, research use only
Technology: Anion exchange chromatography
Brand: NucleoBond
Format: Midi prep
Verpackungseinheit: 2 Prep(s), 20 Prep(s)
Handling: Gravity flow
Automated use: No
Sample material: Animal tissue, Bacteria, Blood, Body fluids, Cells, Human tissue, Plant, Yeast
Sample amount: < 300 mg human/animal tissue, < 10⁷ cultured cells, < 1.5 g plant leaves, < 30 mg bacteria/yeast (ground under liquid nitrogen), < 300 mg bacteria/yeast (lysed enzymatically or by bead beating), < 2 mL liquid sample
Fragment size: 2 kbp–approx. 150 kbp (enzymatic lysis)/50 kbp (mechanical lysis)
Typical yield: Depending on amount and quality of sample
Theoretical binding capacity: 600 µg
Typical purity A260/A280: 1.8–1.9
Elutionsvolumen: 50–250 µL
Preparation time: 2 h/12 preps (incl. 30 min lysis)
Typical downstream application: Long-read sequencing, Next Generation Sequencing
Lagertemperatur: 18−25 °C
Haltbarkeit (ab Produktion): 24 Monate
Gefahrstoffe: Ja