Short DNA Clean-Up Purification Kit

100 Preps

GeneMATRIX Short DNA Clean-Up Purification Kit is designed to isolate DNA fragments, which were subjected to or obtained as a result of various modifications and reactions: PCR products, restriction digests, after kinasing, dephosphorylation, end-trimming/repair, ligation, enzymatic or chemical modification, among others. This kit is especially optimized toward binding oligonucleotides and ds DNA molecules over the range of sizes: from 10 b to 10 kb as well as toward removal of problematic inhibitors of restriction and ligation of DNA. Effectively removed are contaminants such as: nucleotides, ethidum bromide, Taq DNA Polymerase, Pfu DNA Polymerase, endo- and exonucleases, DNA-binding and modifying proteins, BSA and other enzymes/proteins, lipids, dyes, detergents, radio- and chemical labels, EDTA, problematic restriction and ligation inhibitors, buffers and salts. Sample is applied to a DNA binding spin column where DNA molecules are adsorbed to the matrix and contaminants are efficiently washed away. DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.